Association of functional sequence variants of the MYH3 gene with muscle collagen content in pigs

Yong-Jun Kang¹^,⁵, Sang-Hyun Han², Sang-Geum Kim¹^,⁵, Su-Yeon Kim¹, Hyeon-Ah Kim¹^,⁵, Yoo-Kyung Kim¹, Moon-Cheol Shin¹^,⁵, Ji-Hyun Yoo¹, Byoung-Chul Yang¹^,⁵, Hee-Bok Park³, Jun Heon Lee⁴^,⁵^,^*, In-Cheol Cho¹^,⁵^,^**

Author Information & Copyright ▼

¹Subtropical Livestock Research Institute, National Institute of Animal Science, RDA, Jeju 63242, Korea.

²Habitat Conservation Division, Korean National Park Research Institute, Korea National Park Service, Yongju 36015, Korea.

³Department of Animal Resources Science, College of Industrial Sciences, Kongju National University, Kongju 32439, Korea.

⁴Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea.

⁵Reserch Association for Nanchukmacdon, Jeju 63021, Korea.

^**Corresponding Author: Jun Heon Lee, Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea, Republic of. Reserch Association for Nanchukmacdon, Jeju 63021, Korea, Republic of. Phone: +82-42-821-7031. E-mail: junheon@cnu.ac.kr.

^**Corresponding Author: In-Cheol Cho, Subtropical Livestock Research Institute, National Institute of Animal Science, RDA, Jeju 63242, Korea, Republic of. Reserch Association for Nanchukmacdon, Jeju 63021, Korea, Republic of. Phone: +82-64-754-5716. E-mail: choic4753@korea.kr.

© Copyright 2023 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study examined the association between functional sequence variants (FSVs) of <italic>myosin heavy chain 3</italic> (<italic>MYH3</italic>) genotypes and collagen content in a Landrace and Jeju native pig (JNP) crossbred population. Four muscles (<italic>M</italic>.<italic> longissimus dorsi</italic>, <italic>M</italic>. <italic>semimembranosus</italic>, <italic>M</italic>. <italic>triceps brachii,</italic> and <italic>M</italic>. <italic>biceps femoris</italic>) were used for the analysis of meat collagen content, and the same animals were genotyped for the FSVs of the <italic>MYH3</italic> gene by using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Three FSVs of <italic>MYH3</italic> genotypes were identified and had genotype frequencies of 0.358, 0.551, and 0.091 for <italic>QQ,</italic> <italic>Qq,</italic> and <italic>qq</italic>, respectively. <italic>QQ</italic> animals for the FSVs of the <italic>MYH3</italic> genotypes showed higher collagen content in their <italic>M</italic>. <italic>longissimus dorsi </italic>(<italic>p</italic><0.001), <italic>M</italic>. <italic>semimembranosus</italic> (<italic>p</italic><0.001), <italic>M</italic>. <italic>triceps brachii </italic>(<italic>p</italic><0.001), and <italic>M</italic>. <italic>biceps femoris</italic> (<italic>p</italic><0.001) than <italic>qq</italic> homozygous animals. After the validation of this result in other independent populations, the FSVs of <italic>MYH3</italic> genotypes can be a valuable genetic marker for improving collagen content in porcine muscles and can also be applied to increase the amount of collagen for biomedical purposes.

Keywords: Collagen content; Genotype; Muscle; MYH3; Pigs