Complete genome sequence of Ligilactobacillus agilis LDTM47, bacteriocin-producing lactic acid bacteria isolated from broiler gastrointestinal tract

Byeong-Gwan Eum1, Arxel Elnar1, Yujin Jang1, Geun-Bae Kim1,*
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1Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
*Corresponding Author: Geun-Bae Kim, Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea, Republic of. E-mail:

© Copyright 2024 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


<italic>Ligilactobacillus agilis </italic>LDTM47 was isolated from gastric intestinal tract (ileum and jejunum) samples of broiler chickens from a farm associated with Chung-Ang University (Anseong, Korea). <italic>Ligilactobacillus </italic>are Gram-positive lactobacilli generally associated with the intestinal tracts of vertebrates. Members of lactic acid bacteria are considered to have a generally recognized as safe (GRAS) status from the Food and Drug Administration (FDA). The whole genome of <italic>Lig. agilis </italic>LDTM47 was 2,144,466 base pair long assembled into 1 contig, with 2,131 protein-coding sequences, 90 tRNA genes, 24 rRNA genes, and a guanine + cytosine (GC) content of 41.9%. Strain LDTM47 was selected based on its inhibitory activity against <italic>Listeria monocytogenes</italic> during isolation. The genome analysis of LDTM47 revealed genes encoding the bacteriocin core peptides and associated export proteins. Additionally, the stability (instability index, 1.32) and susceptibility of LDTM47 bacteriocin to hydrolysis by proteolytic enzymes (e.g., pepsin, proteinase K, and trypsin) was confirmed <italic>in silico</italic>, suggesting their non-toxicity and potential use as an alternative to antibiotics in controlling pathogenic microorganisms.

Keywords: Ligilactobacillus agilis; postbiotics; bacteriocin; antibiotic alternatives; genome announcement