Article

Complete genome sequence of Enterococcus faecium strain GB_C_05 with potential characteristics applicable as a bacteriocin-producing probiotic feed additive

Juyoun Kang1, Hyunok Doo1, Jinok Kwak1, Eun Sol Kim1,2, Gi Beom Keum1, Yejin Choi1, Haram Kim1, Yeongjae Chae1, Sheena Kim1, Hyeun Bum Kim1,*, Ju-Hoon Lee3,**
Author Information & Copyright
1Department of Animal Biotechnology, Dankook University, Cheonan 31116, Korea.
2Division of Infectious Diseases, Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill 27599, United States.
3Department of Food Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Korea.
*Corresponding Author: Hyeun Bum Kim, Department of Animal Biotechnology, Dankook University, Cheonan 31116, Korea, Republic of. E-mail: hbkim@dankook.ac.kr.
**Corresponding Author: Ju-Hoon Lee, Department of Food Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Korea, Republic of. E-mail: juhlee@snu.ac.kr.

© Copyright 2025 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The whole genome of <italic>Enterococcus faecium</italic> GB_C_05, a strain isolated from Sikhye, a traditional Korean rice beverage, was successfully sequenced and analyzed using Oxford Nanopore Technologies. The complete genome sequence of GB_C_05 contains a circular chromosome with a total length of 2,575,440 base pairs (bp)  and a guanine + cytosine (GC) content of 38.2%, along with one circular plasmid, which has a length of 230,283 bp and a GC content of 35.2%. Annotation of the GB_C_05 genome revealed 2,756 protein-coding sequences (CDSs), 70 tRNAs, and 18 rRNAs on the chromosome. Notably, CDSs related to bacteriocin synthesis (Enterocin) and carbohydrate metabolism (α-galactosidase, β-glucosidase and α-L-arabinofuranosidase), as well as genes potentially involved in probiotic-associated functions such as adhesion and colonization, were identified. This comprehensive study presents the complete genome sequence of <italic>Enterococcus faecium</italic> GB_C_05, providing insight into the diverse additives utilized in animal farming to enhance nutritional quality and livestock productivity.

Keywords: Whole genome sequencing; Enterococcus faecium; Probiotics; Feed additive