Complete genome sequence of Enterococcus faecium strain GB_C_05 with potential characteristics applicable as a bacteriocin-producing probiotic feed additive
Received: Jan 17, 2025; Revised: Apr 06, 2025; Accepted: Apr 06, 2025
Published Online: Apr 23, 2025
Abstract
The whole genome of <italic>Enterococcus faecium</italic> GB_C_05, a strain isolated from Sikhye, a traditional Korean rice beverage, was successfully sequenced and analyzed using Oxford Nanopore Technologies. The complete genome sequence of GB_C_05 contains a circular chromosome with a total length of 2,575,440 base pairs (bp) and a guanine + cytosine (GC) content of 38.2%, along with one circular plasmid, which has a length of 230,283 bp and a GC content of 35.2%. Annotation of the GB_C_05 genome revealed 2,756 protein-coding sequences (CDSs), 70 tRNAs, and 18 rRNAs on the chromosome. Notably, CDSs related to bacteriocin synthesis (Enterocin) and carbohydrate metabolism (α-galactosidase, β-glucosidase and α-L-arabinofuranosidase), as well as genes potentially involved in probiotic-associated functions such as adhesion and colonization, were identified. This comprehensive study presents the complete genome sequence of <italic>Enterococcus faecium</italic> GB_C_05, providing insight into the diverse additives utilized in animal farming to enhance nutritional quality and livestock productivity.
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