Growth performance and blood profiles of Hanwoo steers at fattening stage fed Korean rice wine residue

Seon Ho Kim1,#, Sonny C. Ramos1,#, Chang Dae Jeong1,#, Lovelia L. Mamuad1, Keun Kyu Park2, Yong Il Cho1, Arang Son1, Sang-Suk Lee1,*
Author Information & Copyright
1Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, Korea
2Animal Resources Research Center, School of Animal Life and Science, Konkuk University, Seoul 05029, Korea
*Corresponding author: Sang-Suk Lee, Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, Korea Tel: +82-61-750-3237, E-mail:

#These authors contributed equally to this work.

© Copyright 2020 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jun 12, 2020; Revised: Aug 11, 2020; Accepted: Aug 27, 2020

Published Online: Nov 30, 2020


The aim of this study was to investigate the effects of Korean rice wine residue (RWR) on the growth performance and blood profiles of Hanwoo steers in the fattening stage. In situ and in vivo experiments were conducted to analyze rumen fermentation characteristics and total tract digestibility, respectively. Three cannulated Hanwoo steers (mean body weight: 448 ± 30 kg) were used in both analyses. The growth performance of 27 experimental animals in the fattening stage (initial body weight: 353.58 ± 9.76 kg) was evaluated after 13 months of feeding. The animals were divided into three treatment groups (n = 9/group). The treatments comprised total mixed ration (TMR) only (CON), TMR + 10% RWR (10% RWR), and TMR + 15% RWR (15% RWR). The diets of equal proportions were fed daily at 08:00 and 18:00 h based on 2% of the body weight. The animals had free access to water and trace mineral salts throughout the experiment. Supplementation of 15% RWR significantly decreased (p < 0.05) the rumen fluid pH compared with the control treatment, but there was no significant difference in the total volatile fatty acid concentration. It also significantly increased (p < 0.05) dry matter digestibility compared with the other treatments. The total weight gain and average daily gain of the animals in the RWR-supplemented groups were significantly higher (p < 0.05) than those in the control group. Furthermore, the feed intake and feed efficiency of the RWR-supplemented groups were higher than those of the control group. Supplementation of RWR did not affect the alcohol, albumin, glucose, total cholesterol, triglyceride, and low-density lipoprotein concentrations, and aspartate aminotransferase and alanine transaminase activities in the blood; these parameters were within the normal range. The high-density lipoprotein and creatinine concentrations were significantly higher in the 15% RWR group, whereas the blood urea nitrogen concentration was significantly higher in the 10% RWR group than in the other groups. These results suggest that TMR with 15% RWR can serve as an alternate feed resource for ruminants.

Keywords: In situ; Hanwoo steer; In vivo; Korean rice wine residue


The supply of animal feed is being progressively reduced owing to an increase in human population and various natural phenomena, resulting in a high feed cost. Alternative feedstuffs composed of secondary products obtained during primary commodity processing have been identified [1]. By-product feeds are secondary products of primary product harvesting or processing. The by-products are of nutritional value to humans and animals, and hundreds of by-products are currently used in livestock feed. These by-products are of low cost, which is the principal variable of livestock production. It has been reported that food by-products may have a potential as animal feed because they are rich in crude proteins, cellulose, and water-soluble carbohydrates [2]. Therefore, the main strategies for successful livestock production are reducing feed cost and maintaining productivity. The total mixed ration (TMR) is commonly used in dairy farms and feedlots, as producers aim to reduce cost and increase economic scales [3]. Consequently, by-product feeds are valuable additions to the TMR system [4].

Wet distiller’s by-products (WDB) such as Korean rice wine residues (RWR) can be a protein and energy source in cattle diets [5]. The high energy value of WDB can be attribute to the following reasons: it has three times higher fat content than the usual corn [6]; it enables rapid absorption of ethanol from the rumen, metabolization to acetate, and further utilization for energy or lipogenesis [7,8]; and cattle fed WDB consumed less starch and more corn fiber [9]. Moreover, WDB contains live and dead yeast cells, which show variable effects on ruminal fermentation; it also increases cellulolytic bacterial concentration in the rumen [9,10]. Wet distillers’ grain (WDG) is more economic than dried distillers’ grains with solubles (DDGS) and dried distillers’ grains (DDG) owing to its ability to supply to energy to ruminants and cost efficiency. The WDG lacks a drying process, and therefore, the price of WDG in South Korea (Korean won [KRW] 25 /kg) is lower than that of DDG (KRW 280 /kg) and DDGS (KRW 270 /kg) [5]. Moreover, supplementing 10%–15% RWR could decrease the feed cost to 8%–15% compared with non-supplementation [11]. However, the use of wet by-products is limited by high transportation cost owing to the high moisture content (60%–70%). The shelf-life of wet by-products is typically 3–7 days, but it is related to environmental temperature. Moreover, a sizeable operation is necessary to use a semi-load quantity before the spoilage of wet by-products [12]. Furthermore, the agricultural sector lacks sufficient data on the characteristics and effects of WDG. Kim et al. [5] did not observe any negative effects of up to 10% WDG in the TMR on Hanwoo steer performance. Jeong et al. [11] identified 681 Korean traditional rice wine-processing companies in Korea, producing approximately 83,808 tons of RWR. Rice wine-manufacturing companies usually dispose RWR in landfills or by burning, and thereby causing environmental problems. Thus, strategies to efficiently utilize industrial by-products are necessary. The aim if this study was to evaluate the effects of rice wine residue as a feed substitute on the growth performance of Hanwoo steers in the fattening stage.


Animal care

This study was conducted at the Sunchon National University (SCNU) animal farm and in the Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, SCNU, Jeonnam, South Korea. The animals used in this experiment and all of the experimental protocols were reviewed and approved by the Sunchon National University Animal Research Ethics Committee (SCNU IACUC-2018-01).

Rumen fermentation characteristic (in situ) and total tract digestibility analyses (in vivo)

Three cannulated Hanwoo steers (mean body weight: 448 ± 30 kg) were used in this study. The animals were individually housed in pens and distributed into a 3 × 3 Latin square design. Each period lasted for 24 days (d), with 20 d adaptation and 4 d for the experiment and sampling. The experimental diets (TMR only [CON]; TMR + 10% rice wine residue/dry matter [DM] [10% RWR]; TMR + 15% RWR [15% RWR]) were fed daily in equal proportions at 08:00 and 18:00 based on 2% of the body weight. All of the animals had free access to fresh drinking water and trace mineral salts throughout the experiment. Ruminal fluid was collected from the rumen 6 h after feeding on day 24 of each period. The rumen fluid pH and volatile fatty acids (VFA) were measured via a pH meter (Pinnacle pH meter M540, Corning, NY, USA) and gas chromatography (GC; Varian CP-3800), respectively. Feces were collected daily and immediately stored at −20°C. Rumen DM disappearance of samples was determined according to the protocol of Van Emon et al. [13] with minor modifications. Each samples were dried in an oven at 80°C for 48 h and ground to pass through a 1 mm screen. Ground samples were placed in a nylon bag (5 × 10 cm, 50 ± 10-micron porosity; R510, Ankom Technology, Macedon, NY, USA), and then suspended in the rumen of three cannulated Hanwoo steers. Nylon bags were removed from the rumen after incubation and rinsed in running tap water prior to the next procedure. Bags were again dried in an oven under the same condition as above and residual material was weighed for the determination of rumen DM disappearance [13]. All of the samples were analyzed for total nitrogen, DM, organic matter [14], neutral detergent fiber, and acid detergent fiber [15].

Growth performance and blood profiling of Hanwoo steers in the fattening stage

The Korean rice wine residue (RWR) contains 49% DM, 9.18% crude protein, 0.66% ether extract (EE), 0.88% crude fiber, 0.35% ash, 37.07% nitrogen-free extract, and 5% ethanol. The RWR was stabilized and drained for 48 h before mixing into the animal feed. The TMR was based on the standard feed composition provided by the Korean National Institute of Animal Science (2007). The chemical composition measurements were based on the guidelines of the Association of Official Analytical Chemists [14]. Twenty-seven, Hanwoo steers (initial body weight: 353.58 ± 9.76 kg) were used for 13 months feeding trial. The animals were divided into three treatment groups (nine animals per group) of three experimental diets - TMR only (CON), TMR + 10% RWR (10% RWR), and TMR + 15% RWR (15% RWR). The composition and nutrient content of the TMR containing different RWR inclusion rates (g/kg; DM basis) are shown in Table 1. The steers were housed in steel-constructed pens (5 m × 10 m) with sawdust bedding (three steers per pen) and were marked with numbered ear tags. The TMR diets were fed daily in equal proportions at 08:00 and 18:00 based on 2% of the body weight. The animals also had free access to fresh drinking water and trace mineral salts throughout the experiment. Blood samples were collected at the end of the feeding trial. The in vivo data included the feed intake, weight gain, and average daily gain. Feed efficiency was calculated as the ratio of weight gain to the amount of feed consumed. Five milliliters of blood were taken from the jugular vein of each animal into sterilized vacuum tubes (Green Cross MS, Korea) containing K3-EDTA at the end of the experimental period. The blood samples were centrifuged for 15 min at 890 g at 4°C, and the serum was stored at −20°C until analysis. The contents of total protein (TP), total alcohol, blood urea nitrogen (BUN), albumin, glucose, and creatinine were analyzed using an automated blood serum biochemical analyzer (T Express Plus, USA). The serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglyceride concentrations were analyzed by Green Cross (Yongin, Korea).

Table 1. Composition and nutrient content of the total mixed ration containing Korean rice wine residue at different proportions for Hanwoo steers in the fattening stage
Parameter Treatment1)
CON 10% RWR 15% RWR
Feed composition (% of DM)
 Oat hay 18.87 18.40 19.75
 Timothy hay 5.60 4.91 4.83
 Alfalfa hay 5.62 4.93 4.85
 Rice straw 4.78 4.47 4.58
 Wheat grain 10.09 9.18 8.12
 Corn 19.38 17.87 17.58
 Corn gluten feed 20.73 19.12 15.99
 Lupine 13.38 9.55 6.61
 Salt 0.15 0.14 0.14
 Limestone 0.15 0.15 0.14
 RWR 0.00 10.07 15.04
 Molasses 0.65 0.63 1.77
 Vitamin-mineral mix2) 0.60 0.58 0.58
Nutrient content (% of DM)
 Crude protein 15.20 15.50 15.50
 Ether extract 3.70 5.00 5.50
 Ash 10.40 9.65 9.90
 Crude fiber 18.00 15.90 15.40
 Nitrogen free extract 52.70 53.95 53.70
 Neutral detergent fiber 42.00 41.70 41.30
 Acid detergent fiber 19.54 20.50 19.23
 Total digestible nutrients 76.17 75.54 76.37

1) TMR only (CON); TMR + 10% RWR (10% RWR); TMR + 15% RWR (15% RWR).

2) Vitamin-mineral mix contained vit. A 2,650,000 IU, vit. D3 530,000 IU, vit. E 1,050 IU, niacin 10,000 mg, Mn 4,400 mg, Zn 4,400 mg, Fe 13,200 mg, Cu 2,200 mg, iodine 440 mg, and Co, 440 mg/kg of Grobic-DC provided from Bayer Health Care (Leverkusen, Germany).

RWR, Korean rice wine residue; DM, dry matter; TMR, total mixed ration.

Download Excel Table
Statistical analysis

The data were analyzed using the one-way analysis of variance (ANOVA) followed by analysis with the general linear model with Statistical Analysis Systems (SAS; SAS Inst., Cary, NC, USA) version 9.1 [16]. All treatments were conducted in triplicate. Duncan’s multiple range test and the orthogonal polynomial contrast were used to identify differences among the treatment groups. The differences were considered significant at p < 0.05.


The in situ rumen fermentation characteristics are shown in Table 2. The ruminal pH of the 15% RWR group decreased with time, and it was significantly higher (p < 0.01) than that of the other treatments. In contrast, Jeong et al. [11] reported a higher ruminal pH (p < 0.05) in the control group than in the 15% RWR group after 12 h of incubation. Alcohol promotes the fermentation of carbohydrates by rumen microorganisms, thereby increases the production of various fermented products. The total VFA concentration did not differ among the RWR-supplemented groups, but it tended to increase (p = 0.06) in the 15% RWR group. The lower proportion of propionate among the VFAs during ethanol infusion can be attributed to one or more of the following: inhibition of propionate production during the metabolic process, synthesis of higher chain fatty acids such as valeric acid, and higher absorption of propionate through the rumen wall [17]. Liu et al. [18] reported that branched-chain volatile fatty acids (BCVFA) increased ruminal total VFA, acetate and butyrate, along with the enhanced fiber digestibilities, and microbial enzyme activities. Moreover, supplementation of BCVFA could promote the growth of calf by increasing ruminal butyrate production to improve the rumen development. The increased proportion of butyrate may also be related to an increase in acetate to butyrate conversion in the rumen [1921]. Sutton et al. [21] reported that up to 64% of carbon in butyrate originated from acetate. A slight reduction in the proportion of acetate is possibly a result of increased conversion of acetate to butyrate [21]. A previous study showed that 28% of acetate in the rumen is not absorbed in the form of acetate [22]. Microorganisms can use acetate to produce butyrate through acetyl-CoA transferase and butyryl-CoA transferase [23]. Ruminal microbial metabolism through energy dissipation process continuously converts acetate into butyrate [23,24]. Moreover, acetate delivered by starch into the gastrointestinal tract stimulates the growth of butyrate producing bacteria, and enhances the acetate to butyrate conversion [25]. The results of in vivo total tract digestibility obtained in the present study are shown in Table 3. The DM digestibility in the 15% RWR group was significantly higher than that in the CON group (p < 0.05), but it was not significantly different from that in the 10% RWR group (Table 3). These results are consistent with those of Smith et al. [26], who reported increased DM digestibility with WDGS treatment at different concentrations. Furthermore, Luebbe et al. [27] reported that rumen digestibility and total tract digestibility increased linearly with an increase in the WDG concentration. However, May et al. [28] reported that WDGS from corn and sorghum at different concentrations (from 0% to 30%, DM basis) did not affect the apparent total tract digestibility in feedlot steers.

Table 2. Effects of rice wine residue (RWR) supplementation on the ruminal pH and volatile fatty acid (VFA) concentrations of Hanwoo steers
Parameter Treatment1) SEM p-value
CON 10% RWR 15% RWR
pH 6.28a 6.05b 6.04b 0.06 < 0.01
Total VFA (mM) 57.62 60.25 67.12 2.64 0.06
Acetate (mM) 29.23 29.71 32.94 1.16 0.12
Propionate (mM) 12.28 13.27 13.50 0.78 0.61
Butyrate (mM) 13.71c 14.75b 18.37a 1.06 0.05
Valerate (mM) 0.42c 0.49b 0.64a 0.04 < 0.01
iso-Butyrate (mM) 0.47 0.45 0.39 0.03 0.41
iso-Valerate (mM) 1.50 1.58 1.29 0.08 0.08
A:P ratio 2.39 2.24 2.44 0.11 0.95

1) TMR only (CON); TMR + 10% RWR (10% RWR); TMR + 15% RWR (15% RWR).

a–c Means in each row with different superscripts are significantly different (p < 0.05).

RWR, Korean rice wine residue; VFA, volatile fatty acid; A:P, acetate/propionate ratio; TMR, total mixed ration.

Download Excel Table
Table 3. Effects of rice wine residue supplementation on the total tract digestibility of Hanwoo steers
Digestibility (%) Treatment1) SEM p-value
CON 10% RWR 15% RWR
Dry matter 71.71b 73.90ab 76.25a 0.79 0.05
Crude protein 72.04 72.44 73.58 0.69 0.58
Ether extract 88.85 87.03 88.46 0.58 0.27
Neutral detergent fiber 64.19 62.23 64.17 1.23 0.71
Acid detergent fiber 55.16 55.07 57.61 1.31 0.66

1) TMR only (CON); TMR + 10% RWR (10% RWR); TMR + 15% RWR (15% RWR).

a,b Means in each row with different superscripts are significantly different (p < 0.05).

RWR, Korean rice wine residue; TMR, total mixed ration.

Download Excel Table

The growth performance and blood profiles of Hanwoo steers fed TMR with or without RWR are presented in Tables 4 and 5, respectively. In the fattening stage, the total weight gain and ADG in the 10% RWR (322.86 and 0.82 kg, respectively) and 15% RWR groups (318.14 and 0.81 kg) were significantly higher (p < 0.05) than those in the CON group (273.38 and 0.70 kg). These results suggest that a large amount of ethanol was oxidized to acetate by the rumen microorganisms; it was utilized for the synthesis of VFA of longer chain lengths [17]. Hanson and Ballard [29] reported that acetate is a major precursor for lipogenesis and that adipose tissue is considered the most important site for lipogenesis. Bulumulla et al. [30] reported that rumen VFAs might contribute to meat quality and carcass traits in beef cattle, and this supports the results of total weight gain and ADG in the present study. Yan [31] reported the beneficial effects of ethanol in improving the marbling score of Korean native steers. Furthermore, Lin [32] reported that the production of total volatile acids in the rumen increased whereas that of propionate decreased with the supplementation of alcohol-supplemented feed; this may improve body weight gain in Korean native steers through decreased protein degradation and increased fat synthesis [33]. Firkins et al. [34] fed animals WDG in the diet (50%) and observed a significant increase in the daily gain. The feed efficiency was significantly higher (p < 0.05) in the 10% RWR (0.063) and 15% RWR groups (0.060) than in the CON group (0.053). Alcohol-fermented diets such as RWR are metabolized by the rumen bacteria [35], producing acetate [36] and other VFA. Furthermore, Durix et al. [37] reported that alcohol-fermented diets are degraded to VFAs by the rumen microorganisms. Our feed efficiency results are similar to those of Kristensen et al. [38], who varied the concentration of alcohol (8.7, 5.1, 3.8, and 5.2 MJ/d) in TMR and observed increased feed efficiency in lactating cows. Moreover, researchers have suggested that increased feed efficiency may be partially due to a reduction in subacute acidosis in cattle fed WDG [34,39]. Consequently, subacute acidosis reduces gain and feed efficiency [40] and nutrient absorption [41]. Ham et al. [42] reported that increased energy in distillers by-products, a possible reduction in subacute acidosis, a change in microbial population, and the effects of added moisture are key factors that may account for an increased feed efficiency in cattle. It has been reported that subacute acidosis alleviation is associated with an increase in feed intake [43,44].

Table 4. Effects of rice wine residue supplementation on the growth performance of Hanwoo steers in the fattening stage
Parameter Treatment1) SEM p-value
CON 10% RWR 15% RWR
Growth performance
Initial body weight (kg) 336.16 350.33 338.40 9.76 0.06
Final body weight (kg) 609.54 673.19 656.54 18.15 0.35
Total weight gain (kg) 273.38b 322.86a 318.14a 12.49 0.01
ADG (kg) 0.70b 0.82a 0.81a 00.03 0.03
Feed intake (kg) 5,155.16b 5,084.86a 5,080.96a 34.09 0.04
Feed efficiency 0.05b 0.06a 0.06a 00.00 0.01

1) TMR only (CON); TMR + 10% RWR (10% RWR); TMR + 15% RWR (15% RWR).

a,b Means in each row with different superscripts are significantly different (p < 0.05).

RWR, rice wine residue; ADG, average daily gain; TMR, total mixed ration.

Download Excel Table
Table 5. Effects of rice wine residue supplementation on the blood profiles of Hanwoo steers in the fattening stage
Parameter Treatment1) SEM p-value
CON 10% RWR 15% RWR
Ethanol (%) 0 0 0 0 -
Albumin (g/dL) 3.46 3.64 3.88 0.12 0.47
BUN (mg/dL) 13.38a 11.78b 11.26b 0.84 0.04
Total protein (g/dL) 6.78b 6.71c 6.98a 0.19 0.02
Creatinine (mg/dL) 0.75b 0.91ab 0.99a 0.06 0.04
Glucose (mg/dL) 61.56 64.89 70.88 3.65 0.12
Total cholesterol (mg/dL) 101.78 117.44 124.38 6.75 0.87
Triglyceride (mg/dL) 15.56 20.78 22.25 3.63 0.75
LDL (mg/dL) 15.22 17.67 19.13 1.35 0.64
HDL (mg/dL) 88.22b 102.55ab 109.37a 6.11 0.04
AST/SGOT (U/L) 60.56 60.56 59.88 3.95 0.11
ALT/SGPT (U/L) 24.11 22.67 25.25 1.83 0.18

1) TMR only (CON); TMR + 10% RWR (10% RWR); TMR + 15% RWR (15% RWR).

a–c Means in each row with different superscripts are significantly different (p < 0.05).

RWR, rice wine residue; BUN, blood urea nitrogen; LDL, low density lipoprotein; HDL, high density lipoprotein; AST/SGOT, aspartate aminotransferase/serum glutamic oxaloacetic transaminase; ALT/SGPT, alanine transaminase/serum glutamic pyruvate transaminase; TMR, total mixed ration.

Download Excel Table

Simion et al. [45] also reported normal concentrations of the GPT and GOT enzymes in the blood of cattle. Yan et al. [31] reported higher concentrations of serum triglycerides in steers fed alcohol-fermented feed. However, in this study, RWR did not affect the alcohol, albumin, glucose, total cholesterol, triglyceride, and LDL concentrations in the blood, and aspartate aminotransferase (AST)/SGOT and alanine aminotransferase (ALT)/SGPT activities; these parameters were within the normal range [46]. Low SGOT and SGPT activities are favorable for healthy cattle [46]. In the present study, the HDL and creatinine concentrations in the 15% RWR group (109.37 and 0.99 mg/dL, respectively) was significantly higher (p < 0.05) than those in the other groups. Creatinine is produced as an end product of muscle metabolism and usually eliminated only via the kidneys [47]. Studies have reported that the creatinine concentration increases linearly with the body weight [48,49]. Therefore, the relationship between the creatinine concentration and body weight could follow the same relationship between muscle tissue or crude protein concentration and body weight [50]. These findings explain the increase in the creatinine concentration with RWR supplementation observed in the present study. Steers that showed a high weight gain also produced a high amount of creatinine after blood profile analysis, and this was supported by the findings of Schroeder et al. [48] and De Campeneere et al. [49]. Additionally, Kreul et al. [51] reported improved daily gain and feed efficiency in steers fed diet containing ethanol at 6% of the ration’s DM. A higher energy level of alcohol-containing diets may partially account for the improvement in feedlot performance. Furthermore, Li et al. [52] reported that the addition of a suitable amount of alcohol to the diet of beef cattle can be effective in improving feed efficiency and meat quality. Moreover, Radostits et al. [53] and Smith [54] reported that the cattle blood serum creatinine concentration of up to 1.5 mg/dL is considered normal. Providing alcohol-fermented feed to ruminants increased triglyceride, glucose, and cholesterol concentrations in the blood, and this increased the weight gain [33]. Body fat is correlated with HDL and LDL concentrations; as body weight or fat increases, the HDL and LDL concentrations also increase [46]. Cheeke et al. [55] reported that the designation of HDL is based on the density of blood lipoproteins to which the cholesterol is attached; proteins in solutions have a high density, and therefore HDL has a high protein content. It has been reported that feed concentrates should have a higher level of EE [56], and this supports the results of the present study. The CON group presented the highest (p < 0.05) BUN concentration (13.58 mg/dL). The BUN concentration in cattle is mainly affected by dietary levels of energy and proteins [57]. Roseler et al. [58] suggested that the BUN concentration is an indicator of protein metabolism. The decrease in BUN concentration with the increase in RWR supplementation observed in the present study is supported by the results of Li et al. [52], who reported that 15% alcohol-fermented feed supplementation decreased the BUN concentration. In the present study, although the BUN concentration was significantly lower in the RWR-supplemented treatment groups, the values were still within the normal range. Miller et al. [59] reported that the normal BUN concentration in cattle ranges from 10 to 20 mg/dL. The blood total protein and albumin concentrations in both treatments were within the normal ranges [60]. Serum proteins can be used as an indicator of the nutritional status of an animal, and they constitute a portion of the amino acid pool of the body [61]. Overall, these results show that 15% RWR supplementation can be a substitute feed for ruminants, which will reduce feed costs and increase ADG and total gain.

Competing interests

No potential conflict of interest relevant to this article was reported.

Funding sources

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Advanced Production Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (317016-03-3-WT011).


Not applicable.

Availability of data and material

Upon reasonable request, the datasets of this study can be available from the corresponding author.

Authors’ contributions

Conceptualization: Kim SH, Ramos, SC, Jeong CD, Lee SS.

Data curation: Kim SH, Ramos SC, Jeong CD.

Formal analysis: Kim SH, Ramos SC, Jeong CD, Mamuad LL.

Methodology: Kim SH, Ramos, SC, Jeong CD, Mamuad LL.

Software: Park KK, Cho YI, Son A.

Validation: Lee SS.

Investigation: Kim SH, Ramos SC, Jeong CD.

Writing - original draft: Ramos SC, Jeong CD.

Writing - review & editing: Ramos SC, Jeong CD, Lee SS.

Ethics approval and consent to participate

Animals used in this experiment and all experimental protocols were reviewed and approved by the Sunchon National University Animal Research Ethics Committee (SCNU IACUC-2018-01).



Piao MY, Kim HJ, Seo JK, Park TS, Yoon JS, Kim KH, et al. Effects of synchronization of carbohydrate and protein supply in total mixed ration with Korean rice wine residue on ruminal fermentation, nitrogen metabolism and microbial protein synthesis in holstein steers. Asian-Australas J Anim Sci. 2012; 25:1568-74


Xu C, Cai Y, Moriya N, Ogawa M. Nutritive value for ruminants of green tea grounds as a replacement of brewers’ grains in totally mixed ration silage. Anim Feed Sci Technol. 2007; 138:228-38


Clark E. Midwest becomes battleground for dairy feed market. Feedstuffs. 1992; 64:14-6


Rounds W, Klopfenstein T. Brewers dried grains in ruminant rations. J Anim Sci. 1975; 41:415-6


Kim IY, Ahn GC, Kwak HJ, Lee YK, Oh YK, Lee SS, et al. Characteristics of wet and dried distillers grains on in vitro ruminal fermentation and effects of dietary wet distillers grains on performance of hanwoo steers. Asian-Australas J Anim Sci. 2015; 28:632-8


Zinn RA. Influence of level and source of dietary fat on its comparative feeding value in finishing diets for steers: feedlot cattle growth and performance. J Anim Sci. 1989; 67:1029-37


Tsuda T. Studies on absorption from the rumen II. Absorption of several organic substances from the miniature rumen of the goat. Tohoku J Agric Res. 1957; 7:241-56


Emery RS, Lewis TR, Everett JP, Lassiter CA. Effect of ethanol on rumen fermentation. J Dairy Sci. 1959; 42:1182-6


Larson EM, Stock RA, Klopfenstein TJ, Sindt MH, Huffman RP. Feeding value of wet distillers byproducts for finishing ruminants. J Anim Sci. 1993; 71:2228-36


Dawson KA, Newman KE, Boling JA. Effects of microbial supplements containing yeast and lactobacilli on roughage-fed ruminal microbial activities. J Anim Sci. 1990; 68:3392-8


Jeong CD, Mamuad LL, Ko JY, Sung HG, Park KK, Lee YK, et al. Rumen fermentation and performance of Hanwoo steers fed total mixed ration with Korean rice wine residue. J Anim Sci Technol. 2016; :58-4


Lemenager R, Applegate T, Claeys M, Donkin S, Johnson T, Lake S, et al. The value of distillers’ grains as a livestock feed [Internet]. 2006[cited 2020 Jun 4]Purdue University Cooperative Extension Service


Van Emon ML, Loy DD, Hansen SL. Determining the preference in vitro digestibility, in situ disappearance, and grower period performance of steers fed a novel algae meal derived from heterotrophic microalgae. J Anim Sci. 2015; 93:3121-9


AOAC [Association of Official Analytical Chemists] International. Official methods of analysis of AOAC International. 18th ed Gaithersburg, MD: AOAC International. 2005


Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci. 1991; 74:3583-97


SAS Institute The SAS system for windows. Release 9.0. Cary, NC: SAS Institute. 2002


Pradhan K, Hemken RW. Utilization of ethanol and its effect on fatty acid patterns in ruminants. J Dairy Sci. 1970; 53:1739-46


Liu Q, Wang C, Guo G, Huo WJ, Zhang SL, Pei CX, et al. Effects of branched-chain volatile fatty acids on lactation performance and mRNA expression of genes related to fatty acid synthesis in mammary gland of dairy cows. Animal. 2018; 12:2071-9


Bergman E, Reid R, Murray M, Brockway J, Whitelaw F. Interconversions and production of volatile fatty acids in the sheep rumen. Biochem J. 1965; 97:53-8


Diez-Gonzalez F, Bond DR, Jennings E, Russell JB. Alternative schemes of butyrate production in Butyrivibrio fibrisolvens and their relationship to acetate utilization, lactate production, and phylogeny. Arch Microbiol. 1999; 171:324-30


Sutton JD, Dhanoa MS, Morant SV, France J, Napper DJ, Schuller E. Rates of production of acetate, propionate, and butyrate in the rumen of lactating dairy cows given normal and low-roughage diets. J Dairy Sci. 2003; 86:3620-33


Kristensen NB. Rumen microbial sequestration of [2-13C]acetate in cattle. J Anim Sci. 2001; 79:2491-8


Hackmann TJ, Firkins JL. Maximizing efficiency of rumen microbial protein production. Front Microbiol. 2015; :6-465


Wang L, Zhang G, Li Y, Zhang Y. Effects of high forage/concentrate diet on volatile fatty acid production and the microorganisms involved in VFA production in cow rumen. Animals. 2020; :10-223


Si X, Shang W, Zhou Z, Strappe P, Wang B, Bird A, et al. Gut Microbiome-induced shift of acetate to butyrate positively manages dysbiosis in high fat diet. Mol Nutr Food Res. 2018; :62-1700670


Smith DR, Ponce CH, Dilorenzo N, Quinn MJ, May ML, MacDonald JC, et al. Effects of dietary concentration of wet distillers grains on performance by newly received beef cattle, in vitro gas production and volatile fatty acid concentrations, and in vitro dry matter disappearance. J Anim Sci. 2013; 91:2836-45


Luebbe MK, Patterson JM, Jenkins KH, Buttrey EK, Davis TC, Clark BE, et al. Wet distillers grains plus solubles concentration in steam-flaked-corn-based diets: effects on feedlot cattle performance, carcass characteristics, nutrient digestibility, and ruminal fermentation characteristics. J Anim Sci. 2012; 90:1589-602


May ML, De Clerck JC, Quinn MJ, DiLorenzo N, Leibovich J, Smith DR, et al. Corn or sorghum wet distillers grains with solubles in combination with steam-flaked corn: feedlot cattle performance, carcass characteristics, and apparent total tract digestibility. J Anim Sci. 2010; 88:2433-43


Hanson RW, Ballard FJ. The relative significance of acetate and glucose as precursors for lipid synthesis in liver and adipose tissue from ruminants. Biochem J. 1967; 105:529-36


Bulumulla P, Li M, Chen Y, Li F, White R, Hanigan M, et al. Relationship between ruminal volatile fatty acids and microbial population with carcass and meat quality traits in beef steers. J Anim Sci. 2018; 96(Suppl 3):174


Yan CG. Effects of alcohol-fermented feedstuff on quality meat production in Hanwoo. [Ph.D. dissertation], Chunchon, Korea: Kangwon National University. 1998


Lin GZ. Nutritional metabolism of alcohol-fermented feedstuffs in the rumen of Korean beef cattle. [Ph.D. dissertation], Chunchon, Korea: Kangwon National University. 2001


Lin GZ, Kim BW, Kim CH, Kim HS, Sung KI, Ohh SJ, et al. Changes in serum metabolites and growth characteristics of Korean native steers fed alcohol-fermented feeds. Asian-Australas J Anim Sci. 2004; 17:648-54


Firkins JL, Berger LL, Fahey GC. Evaluation of wet and dry distillers grains and wet and dry corn gluten feeds for ruminants. J Anim Sci. 1985; 60:847-60


Moomaw CR, Hungate RE. Ethanol conversion in the bovine rumen. J Bacteriol. 1963; 85:721-2


Czerkawski JW, Breckenridge G. Fermentation of various glycolytic intermediates and other compounds by rumen micro-organisms, with particular reference to methane production. Br J Nutr. 1972; 27:131-46


Durix A, Jean-Blain C, Sallmann HP, Jouany JP. Use of a semicontinuous culture system (RUSITEC) to study the metabolism of ethanol in the rumen and its effects on ruminal digestion. Can J Anim Sci. 1991; 71:115-23


Kristensen NB, Storm A, Raun BML, Røjen BA, Harmon DL. Metabolism of silage alcohols in lactating dairy cows. J Dairy Sci. 2007; 90:1364-77


Farlin SD. Wet distillers grains. An excellent substitute for corn in cattle finishing rations. Anim Nutr Heal. 1981; :36-35


Stock RA, Sindt MH, Parrott JC, Goedeken FK. Effects of grain type, roughage level and monensin level on finishing cattle performance. J Anim Sci. 1990; 68:3441-55


Huntington GB, Britton RA. Effect of dietary lactic acid on rumen lactate metabolism and blood acid-base status of lambs switched from low to high concentrate diets. J Anim Sci. 1979; 49:1569-76


Ham G, Huffman R, Stock R, Klopfenstein T. Wet corn gluten feed in beef diets. Nebraska Beef Cattle Rep. 1993; 59-A:46-7


Tremere AW, Merrill WG, Loosli JK. Adaptation to high concentrate feeding as related to acidosis and digestive disturbances in dairy heifers. J Dairy Sci. 1968; 51:1065-72


Fulton WR, Klopfenstein TJ, Britton RA. Adaptation to high concentrate diets by beef cattle. I. adaptation to corn and wheat diets. J Anim Sci. 1979; 49:775-84


Simion VE, Pârvu G, Potecea E, Luiza B, Pârvu M. Alteration of some biochemical and haematological parameters in the dairy cows due to the intake of mycotoxin contaminated feeds. Anim Sci Biotechnol. 2010; 43:100-4


Kim SH, Alam MJ, Gu MJ, Park KW, Jeon CO, Ha JK, et al. Effect of total mixed ration with fermented feed on ruminal in vitro fermentation, growth performance and blood characteristics of Hanwoo steers. Asian-Australas J Anim Sci. 2012; 25:213-23


Issi M, Gül Y, Başbuğ O. Evaluation of renal and hepatic functions in cattle with subclinical and clinical ketosis. Turkish J Vet Anim Sci. 2016; 40:47-52


Schroeder AL, Bergen WG, Merkel RA. Estimation of lean body mass, empty body protein and skeletal muscle protein from urinary creatinine excretion in beef steers. J Anim Sci. 1990; :68-311


De Campeneere S, Fiems LO, Boucqué CHV. In vivo estimation of body composition in cattle. Livest Feed Feed. 2000; 70:495-508


Costa e Silva LF, de Campos Valadares Filho S, Chizzotti ML, Rotta PP, Prados LF, Valadares RFD, et al. Creatinine excretion and relationship with body weight of Nellore cattle. Rev Bras Zootec. 2012; 41:807-10


Kreul ML, Yates LD, Gill DR, Dolezal HG, Van Koevering MT. Effects of feeding ethanol on performance and carcass traits of feedlot steers. Oklahoma Agric Exp Stat Anim Sci Res Rep. 1993; :216-22


Li XZ, Park BK, Yan CG, Choi JG, Ahn JS, Shin JS. Effect of alcohol fermented feed on lactating performance, blood metabolites, milk fatty acid profile and cholesterol content in holstein lactating cows. Asian-Australas J Anim Sci. 2012; 25:1546-52


Radostits OM, Gay CC, Hinchcliff KW, Constable PD, Done SH. Veterinary medicine: a textbook of the diseases of cattle, sheep, goats, pigs and horses. 10th ed Edinburgh: Saunders Elsevier. 2007


Smith BP. Large animal internal medicine. 4th ed St. Louis, MO: Mosby Elsevier. 2009


Cheeke PR, Dierenfeld ES. Comparative animal nutrition and metabolism. Wallingford, Oxfordshire: CABI. 2010


Silva PT, Detmann E, Valadares Filho SC, Detmann KSC, Barros LV, Martins SCV, et al. Evaluation of total and non-fatty ether extract in feeds and cattle feces using two analytical methods. Anim Feed Sci Technol. 2011; 163:111-7


Addis T, Barrett E, Poo LJ, Yuen DW. The relation between the serum urea concentration and the protein consumption of normal individuals. J Clin Invest. 1947; 26:869-74


Roseler DK, Ferguson JD, Sniffen CJ, Herrema J. Dietary protein degradability effects on plasma and milk urea nitrogen and milk nonprotein nitrogen in Holstein cows. J Dairy Sci. 1993; 76:525-34


Miller LD, Thoen CO, Throlson KJ, Himes EM, Morgan RL. Serum biochemical and hematologic values of normal and Mycobacterium bovis-infected American bison. J Vet Diagn Invest. 1989; 1:219-22


Seifi HA, Mohri M, Delaramy M, Harati M. Effect of short term over-supplementation of ascorbic acid on hematology, serum biochemistry, and growth performance of neonatal dairy calves. Food Chem Toxicol. 2010; 48:2059-62


Kim JH, Mamuad LL, Yang CJ, Kim SH, Ha JK, Lee WS, et al. Hemato-biochemical and cortisol profile of Holstein growing-calves supplemented with vitamin C during summer season. Asian-Australas J Anim Sci. 2012; 25:361-8

2019 JCR Impact Factor: 1.685

The 2019 Journal Citation Reports (JCR) was announced, and the impact factor of JAST was determined to be 1.685.

We would like to ask for your continued interest and support in our journal.

Thank you.

JAST Editorial Office

I don't want to open this window for a day.