Salmonella is a representative foodborne pathogen which is the most commonly identified in poultry, eggs and dairy products. The most common symptom of salmonella infection is gastroenteritis, follow by bacteremia and enteric fever. Most forms of poultry meat, pork, and beef are the main sources responsible for salmonella infection  because the contamination of the organ and carcass with salmonella easily occurs during the slaughtering process of the food animals at abattoirs .
The Salmonella enterica strain K_SA184 was isolated from a lamb (Ovis aries) purchased from the local traditional market in Suwon, Gyeonggi-do, Korea. The S.enterica strain K_SA184 was streaked to xylose lysine tergitol 4 (XLT4) agar and incubated at 37°C for 24 h. The suspected colony in XLT4 agar was inoculated into Luria-Bertani (LB) broth and incubated at 37°C for 24 h. To analyze the complete genome, the S. enterica strain K_SA184 was sequenced by PacBio RS II (Pacific Biosciences, Menlo Park, CA, USA) at Insilicogen (Yong-in, Korea) and Illumina NextSeq 500 (Illumina, San Diego, CA, USA) platform at LabGenomics (Seongnam, Korea) . The genomic DNA of S. enterica K_SA184 for PacBio and Illumina sequencing were extracted using the MagAttract HMW DNA Kit (QIAGEN), and NucleoSpin® Microbial DNA kit (TAKARA) according to the manufacturer’s instructions. Library preparation was conducted using SMRTbell™ Template Prep Kit 1.0 for Pacbio (Pacific Biosciences) and TruSeq DNA Sample Preparation Kit for Illumina (Illumina) according to the manufacturer’s instructions. PacBio sequencing yielded 1,474,738,487 base pairs and 190,304 long reads after filtering, and 5,513,948 paired-end reads with 832,606,148 bp was obtained with Illumina sequencing. De novo assemble was conducted using the hierarchical genome assembly process (HGAP v2.3.0) workflow and polished using Quiver. Subsequently, Illumina NextSeq reads were aligned to the PacBio RSII assembly using Burrows-Wheeler Aligner (BWA)-MEM v0.7.17-r1188, and the errors were corrected by using Pilon version 1.23 [4,5]. The quality of genome assembly and the validation of the final genome were assessed by using Quality Assessment Tool for Genome Assemblies (QUAST) v5.0.2 and Benchmarking Universal Single-Copy Orthologs (BUSCO) v3.0.2 [6,7].
Open reading frames (ORFs) and RNA genes of S. enterica strain K_SA184 were predicted and functionally annotated by rapid prokaryotic genome annotation (PROKKA) v1.14.5 and Rapid Annotation using Subsystem Technology (RAST) v2.0. The functional categorization and classification of all predicted ORFs were conducted using the RAST server-based SEED viewer and Clusters of Orthologous Groups (COG) – based EggNOG. The putative virulence factors and Antimicrobial resistance were described using BLAST according to the Virulence Factor Database (VFDB) and antibiotic resistome surveillance with the comprehensive antibiotic resistance (CARD) [8,9]. The whole genome of S. enterica strain K_SA184 is composed of one circular chromosome (4,725,087 bp) with 52.3% of guanine + cytosine (G + C) content, 4,363 of coding sequence (CDS), 85 of tRNA, and 22 of rRNA genes.
The complete genome of the S. enterica strain K_SA184 contains the virulence genes encoding Salmonella pathogenicity island 1 & 2 Type III secretion systems which serve several pathogenic functions in killing of macrophages and in interference with immune responses as reported by others . Furthermore, the S. enterica strain K_SA184 also possesses multidrug resistance coding genes which are associated with a variety of drugs resistance Efflux Pumps (mdtk) and Resistance to fluoroquinolones, such as, cephalosporins (AmpC), and fluoroquinolones (Par, Gyr). We summarized the general properties of the S. enterica strain K_SA184’s complete genome in Fig. 1 and Table 1. The further in-vivo studies using S. enterica strain K_SA184 will help us to decipher the potential roles of the virulence genes in the pathogenesis.